This kind of technology is needed more than ever to counter bioterrorism/biowarfare which has become an even more imminent threat now that Iran is motivated to respond with asymmetric warfare and found new support to respond to US bound to the Israeli war, by giving reason for uncertainty in any alliances in the Middle East beyond Israel. Bioterrorism is even more likely now with dismantling means to rapidly respond to infectious disease and blinding our intelligence gathering by alienating our allies. US cannot be an island unto itself. It needs global cooperation to survive.The following is given in more detail, with background references, in Bionanotechnology: Radio Controlled Antimicrobial and Genetic Vectors (Springer-Nature, 2024) and The Black Dragon Trilogy (Amazon eBooks, 2018):
Although the DALM polymer (diazoluminomelanin, poly-hydroxy-phenylene) has the same photon absorption properties as melanin, it is more linear; melanin is stacked oligopolymeric disks.Although DALM is a thermochemiluminescent polymer with a broad radio frequency absorption spectrum, it also demonstrates a strong TADF, historically demonstrated by small organic dyes like eosin. DALM preceded the discovery of any other TADF polymer by 20 years.
The classic thermochemiluminescent response of oxidizing DALM to microwave heating.
After many years of modeling its photochemistry and physical analysis, the above dynamic structure was the final consensus.The thermochemilumiscent response of DALM in a fluid filled hand phantom in a circularly polarized microwave guide. The graph shows the departure of Specific Power Microwave Absorption of formulations of DALM compared to luminol solution.The physical photochemical mechanism of DALMAnthrax bacteria grown on blood agar and then on medium inducing synthesis of DALM, the latter containing the amino acid 3-amino-L-tryosine, nitrate and luminol.Closer look at the coiling phenotype induced by medium that induces DALM synthesis in Bacillus anthracisDALM synthesis in genetically modified human cells (top, HeLa NR1.1) and E. coli bacteria (lower)First evidence of “infectious transfection” of mammalian cells (including human) by DALM synthetic and biosynthetic nanoparticles carrying DNAIron oxide and carbon nanotube components of nanobes demonstrated in cells by TEMAnti-viral activity of nanobe genetic components (vaccinia as a standin for smallpox virus); https://www.cellosaurus.org/CVCL_1277Rapid DALM/microwave killing of resistant sporesDALM nanoparticles attached to the surface of vegetative and spore forms of anthrax bacteria Water flow system to test immobilized DALM nanoparticles ability to kill bacteria Titration to extinction of Bacillus thuringiensis spores added to water system (total 10e10 spores or 10 million spores per ml of water in the system)Demonstration of the magnetic properties of the DALM/magnetite micro/nanoparticles formulation Disk test of nanoparticle antimicrobial activity against Bacillus thuringiensis Antimicrobial action of immobilized DALM nanoparticle against Pseudomonas aeruginosa and then its excreted antibiotic pyocyanin (destroyed by singlet oxygen production by DALM). Pyocyanin is a phenazine pigment.Results in flow system: location and preparations of immobilized nanoparticles After note, yes these nanoparticles can go after intracellular microbes and parasites. The specific fluorescent antibody staining and iron oxide staining indicate the specific anti-rickettsia aptamer on iron nanoparticles transferred a living rickettsia into the target cells where it multiplied.
Global Biosurveillance 